Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression.

• DOI: 10.1128/mcb.13.11.7163
• Publication Date: 2015-10-06T00:36:48Z
• Authors: R R Rivera; M H Stuiver; R Steenbergen; C Murre
• Keywords: Immunoglobulin heavy chain; Basic helix-loop-helix

We used a DNA-protein interaction screening method to isolate cDNA, Erg-3, whose product binds site, designated pi, present in the immunoglobulin (Ig) heavy-chain gene enhancer. Erg-3 is an alternatively spliced of erg and contains Ets DNA-binding domain. Fli-1 PU.1, related proteins, also bind same site. In addition, PU.1 second microB, Ig demonstrate that pi binding site crucial for enhancer function. we show Fli.1, but not can activate reporter construct containing multimer protein-binding sites, synergistically with helix-loop-helix protein E12. discuss how combinatorial interactions between members families may account tissue specificity these proteins.