Chromatin Structure Modulation in Saccharomyces cerevisiae by Centromere and Promoter Factor 1

• DOI: 10.1128/mcb.14.8.5229-5241.1994
• Publication Date: 2021-06-04T19:04:09Z
• Authors: Nicholas A. Kent; Jimmy S. H. Tsang; Daniel J. Crowther; Jane Mellor
• Keywords: Derepression; Bivalent chromatin; ChIA-PET; Chromatin immunoprecipitation

CPF1 is an abundant basic-helix-loop-helix-ZIP protein that binds to the CDEI motif in Saccharomyces cerevisiae centromeres and promoters of numerous genes, including those encoding enzymes methionine biosynthetic pathway. Strains lacking are auxotrophs, it has been proposed might positively influence transcription at MET25 MET16 genes by modulating promoter chromatin structure. We test this hypothesis show regions surrounding motifs maintained a nucleosome-free state requires entire protein. However, structure around does not change on derepression correlate with phenotype cell. An intact but required for transcriptional activation from region upstream sequence. Our results suggest functions modulate these changes do explain how maintain methionine-independent growth. The presence CPF1-dependent structures leads weak repression transcription.