Activator-Specific Requirement of Yeast Mediator Proteins for RNA Polymerase II Transcriptional Activation

• DOI: 10.1128/mcb.19.2.979
• Publication Date: 2015-10-26T10:17:32Z
• Authors: Sang Jun Han; Young Chul Lee; Byung Soo Gim; Gi-Hyuck Ryu; Soon Jung Park; William S. Lane; Young-Joon Kim
• Keywords: RNA polymerase II; Mediator

The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription. composed two subcomplexes, the Rgr1 and Srb4 which appear to function in reception activator signals subsequent modulation Pol activity, respectively. In order determine precise composition explore specific role each protein, our goal was identify all components. To this end, we cloned three previously unidentified subunits, Med9/Cse2, Med10/Nut2, Med11, isolated mutant forms them analyze their transcriptional defects. Differential display Northern analyses mRNAs from wild-type cells demonstrated an activator-specific requirement subunit. Med9/Cse2 Med10/Nut2 were required, respectively, Bas1/Bas2- Gcn4-mediated transcription amino acid biosynthetic genes. Gal11 Gal4- Rap1-mediated activation. Med11 also specifically MFalpha1 On other hand, Med6 these activation processes. These results suggest that distinct proteins subcomplex are mediated by converge on (or subcomplex) modulate activity.