Methylation of Histone H3 by Set2 in Saccharomyces cerevisiae Is Linked to Transcriptional Elongation by RNA Polymerase II

• DOI: 10.1128/mcb.23.12.4207-4218.2003
• Publication Date: 2003-05-28T20:41:15Z
• Authors: Nevan J. Krogan; Minkyu Kim; Amy Tong; Ashkan Golshani; Gerard Cagney; Veronica Canadien; Dawn P. Richards; Bryan K. Beattie; Andrew Emili; Charles Boone; Ali Shilatifard; Stephen Buratowski; Jack Greenblatt
• Keywords: RNA polymerase II; Transcription; Elongation factor; Histone Methylation

Set2 methylates Lys36 of histone H3. We show here that yeast copurifies with RNA polymerase II (RNAPII). Chromatin immunoprecipitation analyses demonstrated and H3 methylation are associated the coding regions several genes were tested correlate active transcription. Both depend, as well, on Paf1 elongation factor complex. The C terminus Set2, which contains a WW domain, is also required for effective methylation. Deletion CTK1, encoding an RNAPII CTD kinase, prevents recruitment, suggesting may be triggered by contact domain or Ser2-phosphorylated CTD. A set2 deletion results in slight sensitivity to 6-azauracil much less beta-galactosidase produced reporter plasmid, resulting from defect In synthetic genetic array (SGA) analysis, growth defects obtained when was combined deletions all five components complex, chromodomain Chd1, putative Soh1, Bre1 Lge1 H2B ubiquitination H2A variant Htz1. SET2 interacts genetically Set1 Set3 complexes, Set1, similarly affect transcription RNAPII.