Support for a Meiotic Recombination Initiation Complex: Interactions among Rec102p, Rec104p, and Spo11p

• DOI: 10.1128/mcb.23.16.5928-5938.2003
• Publication Date: 2003-07-31T17:07:35Z
• Authors: Kai Jiao; Laura Salem; Robert Malone
• Keywords: Recombination, Genetic; 0303 health sciences; Endodeoxyribonucleases; Saccharomyces cerevisiae Proteins; Time Factors; Esterases; Saccharomyces cerevisiae; Models, Biological; Precipitin Tests; DNA-Binding Proteins; Recombinases; Meiosis; 03 medical and health sciences; Two-Hybrid System Techniques; Mutagenesis, Site-Directed; Alleles; Glutathione Transferase; Protein Binding

Initiation of meiotic recombination in the yeast Saccharomyces cerevisiae requires at least 10 gene products. The initiation event creates double-strand breaks, which are then processed by other enzymes. A variety classical observations, such as existence nodules, have suggested that proteins catalyzing form a complex. lines evidence indicate Rad50p, Mre11p, and Xrs2p interact, genetic data suggesting interactions between Rec102p Rec104p been reported. It has recently shown Spo11p coimmunoprecipitates with meiosis well. In this paper, we provide biochemical meiosis-specific Rec102p, Rec104p, all interact each meiosis. Furthermore, demonstrate interaction does not require Rec104p. Likewise, Spo11p, although may stabilize association. suggest trimeric complex during recombination.