A Cellular RNA-Binding Protein Enhances Internal Ribosomal Entry Site-Dependent Translation through an Interaction Downstream of the Hepatitis C Virus Polyprotein Initiation Codon

• DOI: 10.1128/mcb.24.18.7878-7890.2004
• Publication Date: 2004-08-31T00:08:05Z
• Authors: Jong Heon Kim; Ki Young Paek; Sang Hoon Ha; Sungchan Cho; Kobong Choi; Chon Saeng Kim; Sung Ho Ryu; Sung Key Jang
• Keywords: Eukaryotic translation; NS2-3 protease; Coding region; Eukaryotic initiation factor; Eukaryotic Ribosome

Translational initiation of hepatitis C virus (HCV) mRNA occurs by internal entry ribosomes into an ribosomal site (IRES) at the 5′ nontranslated region. A region encoding N-terminal part HCV polyprotein has been shown to augment translation mRNA. Here we show that a cellular protein, NS1-associated protein 1 (NSAP1), augments through specific interaction with adenosine-rich protein-coding within The overexpression NSAP1 specifically enhanced IRES-dependent translation, and knockdown use small interfering RNA inhibited An replicon capable mimicking proliferation process in host cells was further used confirm enhances These results suggest existence novel mechanism translational enhancement acts RNA-binding coding sequence.