Octamer and Sox Elements Are Required for Transcriptional cis Regulation of Nanog Gene Expression

• DOI: 10.1128/mcb.25.6.2475-2485.2005
• Publication Date: 2005-03-02T05:17:42Z
• Authors: Takao Kuroda; Masako Tada; Hiroshi Kubota; Hironobu Kimura; Shin-ya Hatano; Hirofumi Suemori; Norio Nakatsuji; Takashi Tada
• Keywords: Homeobox protein NANOG; POU domain; Histone octamer

The pluripotential cell-specific gene Nanog encodes a homeodomain-bearing transcription factor required for maintaining the undifferentiated state of stem cells. However, molecular mechanisms that regulate expression are largely unknown. To address this important issue, we used luciferase assays to monitor relative activities deletion fragments from 5'-flanking region gene. An adjacent pair highly conserved Octamer- and Sox-binding sites was found be essential activating state-specific expression. Furthermore, 5'-end fragment encompassing Octamer/Sox element sufficient inducing proper green fluorescent protein reporter even in human embryonic (ES) potential OCT4 SOX2 bind verified by electrophoretic mobility shift with extracts F9 embryonal carcinoma cells germ derived day 12.5 embryos. ES cell extracts, complex an undefined preferentially bound element. Thus, may regulated through interaction between Oct4 Sox2 or novel Sox element-binding which is prominent