Normal Cells, but Not Cancer Cells, Survive Severe Plk1 Depletion

• DOI: 10.1128/mcb.26.6.2093-2108.2006
• Publication Date: 2006-03-01T00:02:41Z
• Authors: Xiaoqi Liu; Ming Lei; Raymond L. Erikson
• Keywords: PLK1; HeLa; Mitotic catastrophe

We previously reported the phenotype of depletion polo-like kinase 1 (Plk1) using RNA interference (RNAi) and showed that p53 is stabilized in Plk1-depleted cancer cells. In this study, we further analyzed Plk1 depletion-induced both cells primary The vector-based RNAi approach was used to evaluate role pathway apoptosis with different backgrounds. Although DNA damage cell death can occur independently p53, p53-deficient were much more sensitive than functional p53. Next, lentivirus-based generate a series hypomorphs. HeLa cells, two weak hypomorphs only slight G2/M arrest, medium hypomorph arrested 4N content, followed later by apoptosis, strong underwent serious mitotic catastrophe. well-synchronized level caused 2-h delay progression, high degree significantly delayed entry completely blocked at mitosis. striking contrast, normal hTERT-RPE1 MCF10A less cells; no apparent proliferation defect or cycle arrest observed after these Therefore, data support suggestions may be feasible therapy target.