Fecal metagenomic and metabolomic analyses reveal non-invasive biomarkers of Flavobacterium psychrophilum infection in ayu ( Plecoglossus altivelis )

• DOI: 10.1128/msphere.00301-24
• Publication Date: 2024-06-17T13:00:41Z
• Authors: Mio Takeuchi; Erina Fujiwara-Na...; Kyohei Kuroda; Kenji Sakata; Takashi Narihiro; Jun Kikuchi
• Keywords: Plecoglossus altivelis

ABSTRACT With the rapid growth of inland aquaculture worldwide, side effects such as discharge nutrients and antibiotics pose a threat to global environments. A sustainable future for requires an effective management system, including early detection disease through monitoring specific biomarkers in tanks. To this end, we investigated whether fish feces tanks could be used non-invasive health using ayu ( Plecoglossus altivelis ) infected with Flavobacterium psychrophilum , which causes bacterial cold-water worldwide. Feces that were subsequently produced metagenomic metabolomic analyses. The relative abundances genera Cypionkella (0.6% ± 1.0%, 0.1% 0.2%), Klebsiella (11.2% 10.0%, 6.2% 5.9%), F. (0.5% 0.0% 0.0%) significantly higher infection challenge test than those control cortisol, glucose, acetate 2.4, 1.3 times higher, respectively, Metagenome analysis suggested was by microbes . indicated or metabolites increased after day 4 at earliest, thus considered possible biomarkers. Our results suggest can potentially holistic diseases systems. IMPORTANCE industry is rapidly growing, yet sustainability remains challenge. One crucial task reduce losses due diseases. Monitoring detecting are key establishing aquaculture. Using analyses, found contain various days post-challenge, earliest. findings first step novel, non-invasive, method systems, especially ayu, important freshwater species Asia, promoting future.