Assay Harmonization and Use of Biological Standards To Improve the Reproducibility of the Hemagglutination Inhibition Assay: a FLUCOP Collaborative Study

• DOI: 10.1128/msphere.00567-21
• Publication Date: 2021-07-28T15:34:28Z
• Authors: Joanna Waldock; Lingyi Zheng; Edmond J. Remarque; Alexandre Civet; Branda Hu; Sarah Lartey Jalloh; Rebecca Jane Cox; Sammy Ho; Katja Hoschler; Thierry Ollinger; Claudia Maria Tro...; Othmar G. Engelhardt; Catherine Caillet
• Keywords: 0301 basic medicine; Consensus; Erythrocytes; Turkey; serology; Antibodies, Viral; Microbiology; 333; 03 medical and health sciences; Influenza A Virus, H1N1 Subtype; Influenza, Human; Humans; Intersectoral Collaboration; standardization; hemagglutination inhibition assay; Influenza A Virus, H3N2 Subtype; Reproducibility of Results; Hemagglutination Inhibition Tests; Reference Standards; QR1-502; 3. Good health; hemagglutination inhibition assay; influenza; serology; standardization; Influenza A virus; Influenza Vaccines; influenza; Reassortant Viruses; Research Article

The hemagglutination inhibition (HAI) assay is the most commonly used serology assay to detect antibodies from influenza vaccination or influenza virus infection. This assay has been used for decades but requires improved standardization of procedures to provide meaningful data.