Paenibacillus odorifer, the Predominant Paenibacillus Species Isolated from Milk in the United States, Demonstrates Genetic and Phenotypic Conservation of Psychrotolerance but Clade-Associated Differences in Nitrogen Metabolic Pathways

• DOI: 10.1128/msphere.00739-19
• Publication Date: 2020-01-21T09:36:30Z
• Authors: Sarah M. Beno; Rachel A. Cheng; Renato H. Orsi; Diana R. Duncan; Xiaodong Guo; Jasna Kovac; Laura M. Carroll; Nicole H. Martin; Martin Wiedmann
• Keywords: Spores, Bacterial; 0301 basic medicine; Whole Genome Sequencing; Nitrogen; Cold-Shock Response; Colony Count, Microbial; Genetic Variation; Markov Chains; United States; 03 medical and health sciences; Milk; Phenotype; Nitrogen Fixation; Animals; Paenibacillus; Metabolic Networks and Pathways; Phylogeny; Research Article

Although Paenibacillus species isolates are frequently isolated from pasteurized fluid milk, the link between the genetic diversity and phenotypic characteristics of these isolates was not well understood, especially as some Bacillales isolated from milk are unable to grow at refrigeration temperatures. Our data demonstrate that Paenibacillus spp. isolated from fluid milk represent tremendous interspecies diversity, with P. odorifer being the predominant Paenibacillus sp. isolated. Furthermore, genetic and phenotypic data support that P. odorifer is well suited to transition from a soil-dwelling environment, where nitrogen fixation (and other nitrate/nitrite reduction pathways present only in clade A) may facilitate growth, to fluid milk, where its multiple cold shock-associated adaptations enable it to grow at refrigeration temperatures throughout the storage of milk. Therefore, efforts to reduce bacterial contamination of milk will require a systematic approach to reduce P. odorifer contamination of raw milk.