In Silico Analysis Highlights the Diversity and Novelty of Circular Bacteriocins in Sequenced Microbial Genomes

• DOI: 10.1128/msystems.00047-20
• Publication Date: 2020-06-02T13:55:27Z
• Authors: Bingyue Xin; Hualin Liu; Jinshui Zheng; Chuanshuai Xie; Ying Gao; Dadong Dai; Donghai Peng; Lifang Ruan; Huanchun Chen; Ming Sun
• Keywords: Cereus

Consumer demand for "fresh food" with no chemical preservatives has prompted researchers to pay more attention natural antimicrobial peptides such as bacteriocins. Nisin is currently the most widely used food biopreservative among bacteriocins; however, its applications are restricted due low stability at neutral and alkaline pH values. Circular bacteriocins have potent activity against foodborne pathogens, show exceptional stability, great potential be developed biopreservatives. Here, we take advantage of precursor 15 reported circular devise an in silico approach identify sequenced microbial genomes. A total nearly 7,000 putative were identified from 86 species bacteria further classified into 28 groups based on their amino acid similarity. Among groups, 19 showed similarity (less than 50%) any known peptide One novel bacteriocin group 11, cerecyclin, highest identity (34%) enterocin NKR-5-3B was selected verification. Cerecyclin several Gram-positive bacteria, inhibited outgrowth Bacillus cereus spores, did not exhibit hemolysis activity. Moreover, it 4-fold- 8-fold-higher B. Listeria monocytogenes nisin A. also had increased compared under or conditions. This work only a promising but provided rich source bacteriocins.IMPORTANCE biopreservatives, important enhance current arsenal antimicrobials. In this study, large number these rather 2 that described previous studies. Nineteen finding greatly expands awareness novelty diversity which named cerecyclin group; some pathogens extreme stability. study identification development new