Mosaic Ends Tagmentation (METa) Assembly for Highly Efficient Construction of Functional Metagenomic Libraries

• DOI: 10.1128/msystems.00524-21
• Publication Date: 2021-06-29T14:31:44Z
• Authors: Terence S. Crofts; Alexander G. McFa...; Erica M. Hartmann
• Keywords: Mosaic; Cloning (programming)

Medically and industrially important genes can be recovered from microbial communities by high-throughput sequencing, but precise annotation is often limited to characterized their relatives. Cloning a metagenome en masse into an expression host produce functional metagenomic library, directly connecting functions, sequence-naive cultivation-independent method discover novel genes.