Dairy Products and Dairy-Processing Environments as a Reservoir of Antibiotic Resistance and Quorum-Quenching Determinants as Revealed through Functional Metagenomics

• DOI: 10.1128/msystems.00723-19
• Publication Date: 2020-02-17T13:09:31Z
• Authors: Elena A. Alexa (O...; Calum J. Walsh; Laura M. Coughlan; Amal Awad; Cezara A. Simon; Lorena Ruiz; Fiona Crispie; Paul D. Cotter; Avelino Alvarez-O...
• Keywords: Quorum Quenching

The study shows the potential of functional metagenomics analyses to uncover diversity functions in microbial communities prevailing dairy products and their processing environments, evidencing that lactic acid bacteria (LAB) dominate cheese microbiota, whereas Gram-negative microorganisms animal or soil origin microbiota milk cheese-processing environments. silico screening library allowed identification LAB, especially Lactococcus lactis , as a relevant reservoir antimicrobial resistance (AR) determinants cheese. Quorum-quenching (QQ) were not recovered through execution wet-lab function-based screenings but detected sequencing-based analyses.