Comparative Molecular and Immunoregulatory Analysis of Extracellular Vesicles from Candida albicans and Candida auris

• DOI: 10.1128/msystems.00822-21
• Publication Date: 2021-08-24T13:31:13Z
• Authors: Daniel Zamith-Mir...; Heino M. Heyman; Sneha P. Couvillion; Radames J. B. Cor...; Marcio L. Rodrigues; Leonardo Nimrichter; Arturo Casadevall; Rafaela F. Amatuzzi; Lysangela R. Alves; Ernesto S. Nakayasu; Joshua D. Nosanchuk
• Keywords: Candida auris

Candida auris is a recently described multidrug-resistant pathogenic fungus that increasingly responsible for health care-associated outbreaks across the world. Bloodstream infections of this cause death in up to 70% cases. Aggravating scenario, disease-promoting mechanisms C. are poorly understood. Fungi release extracellular vesicles (EVs) carry broad range molecules, including proteins, lipids, carbohydrates, pigments, and RNA, many which virulence factors. Here, we carried out comparative molecular characterization albicans EVs evaluated their capacity modulate effector host immune defense. Using proteomics, lipidomics, transcriptomics, found released with payloads were significantly different from those by albicans. potentiated adhesion yeast an epithelial cell monolayer, while had no effect. primed macrophages enhanced intracellular killing, whereas promoted survival fungal cells. Moreover, both induced activation bone marrow-derived dendritic Together, our findings show distinct profiles properties highlight potential contribution pathogenesis emerging pathogen. IMPORTANCE globe, particularly context nosocomial infections. Its factors tested hypothesis factor. We describe production compare biological activities against better-characterized have immunoregulatory properties, some opposite EVs. also explored cargo structural components they remarkably compared auris's phylogenetic relative