Deciphering the Microbial Taxonomy and Functionality of Two Diverse Mangrove Ecosystems and Their Potential Abilities To Produce Bioactive Compounds

• DOI: 10.1128/msystems.00851-19
• Publication Date: 2020-10-26T13:08:55Z
• Authors: Shuilin Liao; Yayu Wang; Huan Liu; Guangyi Fan; Sunil Kumar Sahu; Tao Jin; Jianwei Chen; Pengfan Zhang; Lone Gram; Mikael Lenz Strube; Qiong Shi; Simon Ming Yuen Lee; Xin Liu
• Keywords: Resistome

Mangroves, as important and special ecosystems, create unique ecological environments for examining the microbial gene capacity potential producing bioactive compounds. However, little is known about biogeochemical implications of microbiomes in mangrove especially variations between pristine anthropogenic mangroves. To elucidate this, we investigated taxonomic functional shifts their compounds two different coastal ecosystems southern China. A catalogue, including 87 million genes, was constructed, based on deep shotgun metagenomic sequencing. Differentially enriched bacterial archaeal taxa mangroves (Guangxi) (Shenzhen) were found. The Nitrospira ammonia-oxidizing archaea, specifically, more abundant Shenzhen mangroves, while sulfate-reducing bacteria methanogens Guangxi results analysis consistent with results, indicating that microbiome has a higher abundance genes involved nitrogen metabolism sulfur methanogenesis. Biosynthetic clusters identified metagenome data hundreds de novo reconstructed nonredundant genomes, respectively. Notably, found biosynthetic taxa, three high quality novel Acidobacteria genomes large number BGCs. In total, 67,278 annotated antibiotic resistance, prevalence persistence multidrug-resistant microbiome.IMPORTANCE This study comprehensively described taxonomy functionality microbiomes, secondary metabolite biosynthesis ability to resist antibiotics. characteristics differed geographical locations, corresponding environmental condition diverse regions. encoding bioactivities found, this can serve valuable resource guide compound discovery clinical uses.