A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

• DOI: 10.1128/msystems.01222-20
• Publication Date: 2021-02-15T13:53:03Z
• Authors: Susan Joseph; Joseph Aduse-Opoku; Ahmed Hashim; Eveliina Hanski; Ricarda Streich; Sarah C. L. Knowles; Amy B. Pedersen; William G. Wade; Michael A. Curtis
• Keywords: Oral Microbiome

A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The comprises collection nearly full-length sequences from cultured isolates draft genomes representative taxa collected range sources, including specific-pathogen-free laboratory mice, wild Mus musculusdomesticus formerly wood mouse Apodemus sylvaticus At present, it 103 (MOT) spanning four phyla-Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes-including 12 novel undescribed species-level taxa. key observations this study are (i) the low diversity predominantly culturable nature (ii) identification three major murine-specific lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), Gemella species 2 (MOT43), which is one novel, still-unnamed Of these, S. particular interest, since component all strains healthy periodontally diseased well being present in mice. It expected that well-characterized should useful resource vitro experimentation model field microbiology.IMPORTANCE Mouse frequently research, particularly investigate diseases such periodontitis caries, other related systemic diseases. We have reported here details development characterize microbial community some genomic information findings can help improve outcomes accuracy host-microbe experimental use models understand health disease. Work also under way make data sets publicly available on web server enable easy access downloading researchers across world.