Association of tumor microbiome with survival in resected early-stage PDAC

• DOI: 10.1128/msystems.01229-24
• Publication Date: 2025-02-27T14:00:34Z
• Authors: Yixuan Meng; Chan Wang; Mykhaylo Usyk; Soyoung Kwak; Chengwei Peng; Kenneth S. Hu; Paul E. Oberstein; Michelle Krogsgaard; Huilin Li; Richard B. Hayes; Jiyoung Ahn

ABSTRACT The pancreas tumor microbiota may influence microenvironment and survival in early-stage pancreatic ductal adenocarcinoma (PDAC); however, current studies are limited small. We investigated the relationship of to 201 surgically resected patients with localized PDAC (Stages I–II), from Cancer Genome Atlas (TCGA) International Consortium (ICGC) cohorts. characterized microbiome using RNA-sequencing data. examined association overall (OS), via meta-analysis Cox PH model. A microbial risk score (MRS) was calculated OS-associated microbiota. further explored whether is related host immune infiltration. α- β-diversities were not associated OS; 11 bacterial species, including species Gammaproteobacteria , confirmed by extensive resampling, significantly OS (all Q < 0.05). MRS summarizing these bacteria a threefold change (hazard ratio = 2.96 per standard deviation MRS, 95% confidence interval 2.26–3.86). This result consistent across two cohorts stratified analyses adjuvant therapy (chemotherapy/radiation). Identified also exhibited memory B cells naïve CD4 + T cells, which be landscape through BCR TCR signaling pathways. Our study shows that unique structure, potentially affecting microenvironment, poorer PDAC. These findings suggest mechanisms involved survival, informing prognosis guiding personalized treatment strategies. IMPORTANCE Much available data on derived relatively small heterogeneous studies, those involving advanced stages cancer. There critical knowledge gap terms treated surgical resection; we expect advancements initially best achieved who curative intent.