Gut commensal Alistipes shahii improves experimental colitis in mice with reduced intestinal epithelial damage and cytokine secretion

• DOI: 10.1128/msystems.01607-24
• Publication Date: 2025-02-12T14:00:42Z
• Authors: Xiaoying Lin; Mingchao Xu; Ruiting Lan; Dalong Hu; Suping Zhang; Shuwei Zhang; Yao Lu; Hui Sun; Jing Yang; Liyun Liu; Jianguo Xu
• Keywords: Intestinal mucosa

ABSTRACT The commensal bacterium Alistipes shahii is a core microbe of the human gut microbiome and its abundance negatively correlated with inflammatory bowel diseases (IBDs). However, fundamental role in regulating response remains unknown. Using dextran sulfate sodium (DSS)-induced colitis mouse model, we examined effect A. strain As360 intervention on host found that alleviated disease activity index, colon shortening, colonic histopathological lesion. levels tight junction proteins (mainly ZO1 claudin-1) were decreased DSS-induced mice, whereas these elevated mice treatment. In addition, treatment led to alterations cytokine release, especially an increase IL10. It also reduced expressions mtor Nlrp3 increased expression mTOR inhibitor Ddit4 at transcriptional level. 16S rRNA amplicon sequencing Bacteroides , producer short-chain fatty acids (SCFAs), was enriched fecal samples Metabolic analyses that, following treatment, SCFAs content lactic acid cecal content. These findings suggest supplementation promising strategy prevent colitis. IMPORTANCE As one microbes keystone species gut, has potential inhibit inflammation improve (IBDs) conditions. this study, experimentally demonstrated oral administration symptoms colitis, altered release cellular factors, intestinal epithelial barrier damage, changed microbiota metabolites. provide deeper understanding beneficial effects perspective for better strategies IBD.