Gut Microbiota-Testis Axis: FMT Mitigates High-Fat Diet-Diminished Male Fertility via Improving Systemic and Testicular Metabolome

• DOI: 10.1128/spectrum.00028-22
• Publication Date: 2022-04-21T13:02:34Z
• Authors: Yanan Hao; Yanni Feng; Xiaowei Yan; Liang Chen; Xiangping Ma; Xiangfang Tang; Ruqing Zhong; Zhongyi Sun; Manjree Agarwal; Hongfu Zhang; Yong Zhao
• Keywords: Metabolome; Semen quality; Semen Analysis

High-fat diet (HFD)-induced obesity is known to be associated with reduced male fertility and decreased semen quality in humans. HFD-related infertility a growing issue worldwide, it crucial overcome this problem ameliorate the distress of infertile couples. For first time, we discovered that fecal microbiota transplantation (FMT) alginate oligosaccharide (AOS)-improved gut (A10-FMT) ameliorated HFD-decreased (sperm concentration: 286.1 ± 14.1 versus 217.9 17.4 million/mL; sperm motility: 40.1 0.7% 29.0 0.9%), (pregnancy rate: 87.4 1.1% 70.2 6.1%) by benefiting blood testicular metabolome. A10-FMT improved HFD-disturbed increasing Bacteroides (colon: 24.9 8.3 0.6%; cecum: 10.2 3.6 0.7%) decreasing Mucispirillum 0.3 0.1% 2.8 0.4%; 2.3 0.5% 6.6 0.7%). benefited improve liver function adjusting lipid metabolism produce n-3 polyunsaturated fatty acids, such as eicosapentaenoic acid (blood: 55.5 18.7 20.3 2.4) docosahexaenoic (testis: 121.2 6.2 89.4 6.7), thus ameliorating HFD-impaired microenvironment rescue spermatogenesis increase fertility. The findings indicated AOS-improved may promising strategy treat or metabolic issues-related future. IMPORTANCE HFD decreases via upsetting AOS-benefited improves HFD-reduced Moreover, benefit metabolome simultaneously turn process on. We demonstrated could applied males issues induced HFD.