Bacteroides vesicles promote functional alterations in the gut microbiota composition

• DOI: 10.1128/spectrum.00636-24
• Publication Date: 2024-09-30T13:11:42Z
• Authors: Olga Yu. Shagaleeva; Daria A. Kashatni...; Dmitry A. Kardonsky; Boris A. Efimov; Viktor A. Ivanov; Svetlana V. Smirnova; Suleiman S. Evsiev; Eugene A. Zubkov; Olga V. Abramova; Yana A. Zorkina; Anna Y. Morozova; Elizaveta A. Voro...; Artemiy S. Silantiev; Irina V. Kolesnikova; Maria I. Markelova; Evgenii I. Olekhn...; Maxim D. Morozov; Polina Y. Zoruk; Daria I. Boldyreva; Victoriia D. Kaza...; Anna A. Vanyushkina; Andrei V. Chaplin; Tatiana V. Grigor...; Natalya B. Zakhar...
• Keywords: Bacteroides fragilis; Metabolome; Dysbiosis; Bacteroidaceae

ABSTRACT Inflammatory bowel diseases are characterized by chronic intestinal inflammation and alterations in the gut microbiota composition. Bacteroides fragilis , which secretes outer membrane vesicles (OMVs) with polysaccharide A (PSA), can moderate inflammatory response possibly alter In this study, we created a murine model of sodium dextran sulfate (DSS)-induced colitis treated it B. OMVs. We monitored efficiency OMV therapy determining disease activity index (DAI) performing histological examination (HE) intestine before after vesicle exposure. also analyzed composition using 16S rRNA gene sequencing. Finally, evaluated volatile compound animals’ stools HS-GC/MS to assess functional microbiota. observed more effective repair treatment according DAI HE. metabolomic study revealed changes microbiota, predominance phenol pentanoic acid control group compared DSS OMVs (DSS OMVs). positive correlation these metabolites Saccharibacteria Acetivibrio group, whereas there was negative Lactococcus Romboutsia . According metabolome sequencing data, DSS-treated intermediate between that groups. not only have an anti-inflammatory effect but contribute recovery IMPORTANCE contain superficially localized has unique immune-modulating properties. Isolated PSA prevent chemically induced model. Outer digestive enzymes complement properties PSA. showed high therapeutic against sulfate-induced colitis, as confirmed assays. fecal samples from different stages, supplemented comprehensive analysis compounds conducted HS-GC/MS, structural under influence Correlation OMV-treated untreated experimental animal groups associations