Limit of detection of Salmonella ser. Enteritidis using culture-based versus culture-independent diagnostic approaches

• DOI: 10.1128/spectrum.01027-24
• Publication Date: 2024-11-04T15:19:15Z
• Authors: L. M. Bradford; L. Yao; C. Anastasiadis; A. L. Cooper; B. Blais; A. Deckert; R. Reid-Smith; C. Lau; M. S. Diarra; C. Carrillo; A. Wong
• Keywords: Salmonella enteritidis; Microbiological culture; Enrichment culture

To prevent the spread of foodborne illnesses, presence pathogens in food chain is monitored by government agencies and producers. The culture-based methods currently employed are sensitive but time- labor-intensive, leading to increasing interest exploring culture-independent diagnostic tests (CIDTs) for pathogen detection. However, few studies quantify relative sensitivity reliability these CIDTs compared current approaches. address this issue, we conducted a comparison limit detection (LOD