Evaluation of human antibodies from vaccinated volunteers for protection against Yersinia pestis infection

• DOI: 10.1128/spectrum.01054-24
• Publication Date: 2024-08-27T13:01:50Z
• Authors: Li Zhang; Binyang Zheng; Jing Lu; Haisheng Wu; Hailian Wu; Qi Zhang; Lei Jiao; Hongxing Pan; Jianfang Zhou
• Keywords: Yersinia pestis; Yersiniosis

has a broad host range and caused lethal bubonic pneumonic plague in humans. With the emergence of multiple resistant strains potential for biothreat use, there is an urgent need new therapeutic strategies that can protect populations from natural or deliberate infection. Targeting F1 been proven to be main strategy developing vaccines antibodies, but data on anti-F1 especially humans, are scarce. To date, three human monoclonal antibodies (m252, αF1Ig2, αF1Ig8) naive have reported. Here, we constructed antibody library vaccinees immunized with subunit vaccine IIa by phage display. The genetic basis, epitopes, biological functions obtained mAbs were assessed evaluated plague-challenged mice. Three mAbs, namely, F3, F19, F23, identified. Their biolayer responses 0.4, 0.6, 0.6 nm, respectively. dissociation constants (K