Lipid and Nucleocapsid N-Protein Accumulation in COVID-19 Patient Lung and Infected Cells

• DOI: 10.1128/spectrum.01271-21
• Publication Date: 2022-02-16T15:30:59Z
• Authors: Anita E. Grootemaat; Sanne van der Niet; Edwin R. Scholl; Eva Roos; Bernadette Schurink; Marianna Bugiani; Sara E. Miller; Per Larsen; Jeannette Pankras; Eric A. Reits; Nicole N. van der...
• Keywords: Male; 0301 basic medicine; Adolescent; Fluorescent Antibody Technique; Microbiology; Disease Outbreaks; lipids; 03 medical and health sciences; Chlorocebus aethiops; Animals; Humans; Microscopy, Immunoelectron; Nucleocapsid; Lung; Aged; electron microscopy; SARS-CoV-2; COVID-19; Middle Aged; Lipid Metabolism; Immunohistochemistry; Lipids; QR1-502; 3. Good health; Child, Preschool; pathology; Female; Rabbits; Research Article

Visualization of the subcellular localization of SARS-CoV-2 proteins in lung patient material of COVID-19 patients is important for the understanding of this new virus. We detected viral proteins in the context of the ultrastructure of infected cells and tissues and discovered that some viral proteins accumulate in novel, lipid-filled compartments.