Functional Characterization of FeoAB in Iron Acquisition and Pathogenicity in Riemerella anatipestifer

• DOI: 10.1128/spectrum.01373-23
• Publication Date: 2023-06-05T14:00:12Z
• Authors: Mi Huang; Mengying Wang; Yan Feng; Mingshu Wang; Qun Gao; Dekang Zhu; Renyong Jia; Shun Chen; Xinxin Zhao; Qiao Yang; Ying Wu; Shaqiu Zhang; Bin Tian; Juan Huang; Xumin Ou; Sai Mao; Di Sun; Yu He; Zhen Wu; Anchun Cheng; Mafeng Liu
• Keywords: Strain (injury); Colony-forming unit

The bacterium Riemerella anatipestifer requires iron for growth, but the mechanism of uptake is not fully understood. In this study, we disrupted Feo system and characterized its function in import R. ATCC 11845. Compared to parent strain, growth ΔfeoA, ΔfeoB, ΔfeoAB strains was affected under Fe3+-limited conditions, since absence feo led less intracellular than strain. parallel, strain shown be sensitive streptonigrin, an antibiotic that free function. sensitivity hydrogen peroxide also observed diminished compared with which could related reduced content Further research revealed feoA feoB were directly regulated by through Fur regulator transcript levels significantly increased medium supplemented 1 mM MnCl2, 400 μM ZnSO4, 200 CuCl2. Finally, it 11845 impaired ability colonize blood, liver, brain ducklings. Taken together, these results demonstrated FeoAB supports ferrous acquisition plays important role colonization. IMPORTANCE Gram-negative bacteria, transport system. encodes system, unknown. As may required oxidative stress protection virulence, understanding contribution transporters processes crucial. This study showed debilitated constitutively low, enhanced resistance deficient H2O2. We surprised find that, addition responding iron, play sensing manganese, zinc, copper stress. colonization sheds light on host-pathogen interactions. cross talk between other metal pathways, as well pathogenic anatipestifer.