Molecular characterization and descriptive analysis of carbapenemase-producing Gram-negative rod infections in Bogota, Colombia

• DOI: 10.1128/spectrum.01714-23
• Publication Date: 2024-04-17T13:02:26Z
• Authors: Elsa D. Ibáñez-Prada; Ingrid G. Bustos; Enrique Gamboa-Silva; Diego F. Josa; Lina Mendez; Yuli V. Fuentes; Cristian C. Serra...; Oscar Baron; Alejandra Ruiz-Cu...; Edwin Silva; Louise M. Judd; Taylor Harshegyi; Hector F. Africano; Juan Urrego-Reyes; Claudia C. Beltran; Sebastian Medina; Rafael Leal; Andrew J. Stewardson; Kelly L. Wyres; Jane Hawkey; Luis Felipe Reyes
• Keywords: Gram; Gram-Negative Bacteria

ABSTRACT In this study, the genetic differences and clinical impact of carbapenemase-encoding genes among community healthcare-acquired infections were assessed. This retrospective, multicenter cohort study was conducted in Colombia included patients infected with carbapenem-resistant Gram-negative rods between 2017 2021. Carbapenem resistance identified by Vitek, whole-genome sequencing (WGS) to classify alleles sequence types (STs). Descriptive statistics used determine association any pathogen or gene outcomes. A total 248 included, which only 0.8% (2/248) had community-acquired infections. Regarding bacteria, most prevalent pathogens Pseudomonas aeruginosa Klebsiella pneumoniae . WGS analysis, 228 isolates passed all quality criteria analyzed. The principal bla KPC, specifically KPC-2 [38.6% (88/228)] KPC-3 [36.4% (83/228)]. These frequently detected co-concurrence VIM-2 NDM-1 Notably, allele [50.0% (1/2)]. reference STs, 78 identified, ST111 mainly related blaKPC-3. ST512, ST258, ST14, ST1082 exclusively associated KPC-3. Finally, no particular worse carbapenemase-producing KPC-3, both co-occurrence diverse STs healthcare environment. Patients several systemic complications poor outcomes that not a gene. IMPORTANCE Antimicrobial is pandemic worldwide public health problem, especially carbapenem low- middle-income countries. Limited data regarding molecular characteristics these bacteria are available. Thus, our described community- identified. We also found distinct types, two novel , could represent challenges treating Our shows low countries, such as Colombia, burden concern for health, regardless allele, studies assessing local epidemiology, prevention strategies (including trials), underpinning mechanisms urgently needed,