A High-Throughput Yellow Fever Neutralization Assay

• DOI: 10.1128/spectrum.02548-21
• Publication Date: 2022-06-07T13:02:36Z
• Authors: Madina Rasulova; Thomas Vercruysse; Jasmine Paulissen; Catherina Coun; Vanessa Suin; Leo Heyndrickx; Ji Ma; Katrien Geerts; Jolien Timmermans; Niraj Mishra; Li-Hsin Li; Dieudonné Buh Kum; Lotte Coelmont; Steven Van Gucht; Hadi Karimzadeh; Julia Thorn-Seshold; Simon Rothenfußer; Kevin K. Ariën; Johan Neyts; Kai Dallmeier; Hendrik Jan Thibaut
• Keywords: HEPATITIS-A; IMMUNOGENICITY; MEASLES; Antibodies, Viral; Japanese Encephalitis Virus ; Prnt ; Zika Virus ; Dengue Virus ; High-throughput ; Neutralization Assay ; Reporter Virus ; Serodiagnosis ; Yellow Fever Virus; Microbiology; Zika virus; reporter virus; 03 medical and health sciences; neutralization assay; Neutralization Tests; Yellow Fever; IMMUNE-RESPONSE; Humans; Encephalitis, Japanese; Biology; high-throughput; yellow fever virus; INTERFERENCE; serodiagnosis; 0303 health sciences; Science & Technology; IGM; dengue virus; Zika Virus Infection; PRNT; Methods and Protocols; Zika Virus; Antibodies, Neutralizing; QR1-502; 3. Good health; Japanese encephalitis virus; SAFETY; VIRUS; Yellow fever virus; Life Sciences & Biomedicine; COMBINED VACCINE

Fast and accurate detection of neutralizing antibodies (nAbs) against yellow fever virus (YFV) is key in serodiagnosis, outbreak surveillance, monitoring vaccine efficacy. Although classical PRNT remains the gold standard for measuring YFV nAbs, this methodology suffers from inherent limitations such as low throughput overall high labor intensity.