Probiotics Mediate Intestinal Microbiome and Microbiota-Derived Metabolites Regulating the Growth and Immunity of Rainbow Trout (Oncorhynchus mykiss)

• DOI: 10.1128/spectrum.03980-22
• Publication Date: 2023-03-14T14:15:35Z
• Authors: Chunyan Zhao; Xianhui Men; Yongji Dang; Yangen Zhou; Yichao Ren
• Keywords: Intestinal Microbiome

Emerging evidence confirms using probiotics in promoting growth and immunity of farmed fish. However, the molecular mechanisms underlying host-microbiome interactions mediated by are not fully understood. In this study, we used rainbow trout (Oncorhynchus mykiss) as a model to investigate internal influenced two probiotic bacteria, Bacillus velezensis Lactobacillus sakei. We carried out experiments, including intestinal histology, serum physiology, transcriptome combined microbiome metabolite profiling. Our results showed that both had positive effect on growth, immunity, enzyme activity, gut microbiome, resistance Aeromonas salmonicida trout. Moreover, microbial structure was reshaped with increased relative abundance potential beneficial such Ruminococcus, Lachnospiraceae ucg-004, Leptotrichia, coagulans, Porphyromonadaceae, Anaerococcus, Photobacterium B. group Paenibacillaceae Eubacterium hallii L. sakei group. Metabolomic profiling analysis revealed upregulated metabolites biomarkers, i.e., sucrose l-malic acid group, N-acetyl-l-phenylalanine, N-acetylneuraminic acid, hydroxyproline Additionally, multiomics illustrated significant correlations between microflora, metabolites, gene expression associated growth. This study highlights role effectors activity shows different can have species-specific physiological regulation host. These findings contribute better understanding complex may implications for use aquaculture. IMPORTANCE Probiotics kinds live microbes impart effects Recent studies proven when given supplementation probiotics, fish improved disease prevention promotion. metabolic functions regarding their involvement regulating phenotypes, nutrient utilization, immune response yet well understood aquaculture field. Given active microbiota performance, conducted experiments The microbiome- microbiota-derived fish, participated contributed functional host health species.