Antifungal potential of lipopeptides produced by the Bacillus siamensis Sh420 strain against Fusarium graminearum

• DOI: 10.1128/spectrum.04008-23
• Publication Date: 2024-03-07T15:26:21Z
• Authors: Sarfaraz Hussain; Bowen Tai; Maratab Ali; Israt Jahan; Suha Sakina; Gang Wang; Xinlong Zhang; Yixuan Yin; Fuguo Xing
• Keywords: Lipopeptide; Beauvericin; Ergosterol; Strain (injury); Surfactin

ABSTRACT Biological control is a more sustainable and environmentally friendly alternative to chemical fungicides for controlling Fusarium spp. infestations. In this work, Bacillus siamensis Sh420 isolated from wheat rhizosphere showed high antifungal activity against graminearum as secure substitute fungicides. was identified B. using phenotypic evaluation 16S rDNA gene sequence analysis. An in vitro antagonistic study that Sh420’s lipopeptide (LP) extract exhibited strong properties effectively combated F. . Meanwhile, lipopeptides have the ability decrease ergosterol content, which has an impact on overall structure stability of plasma membrane. The PCR-based screening revealed presence LP biosynthetic genes strain’s genomic DNA. crude Sh420, we were able discover several LPs such bacillomycin, iturins, fengycin, surfactins ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry. Microscopic investigations (fluorescent/transmission electron microscopy) deformities alterations morphology phytopathogen upon interaction with LPs. been shown grape tests be effective infection stimulate antioxidant fruits by avoiding rust gray lesions. findings highlight potential biological agent IMPORTANCE This addresses extracts obtained strain Sh420. isolate acts crucial player providing suppressing phytopathogen. Moreover, these can reduce content influencing its PCR provided confirmation regarding existence responsible biosynthesizing DNA Several antibiotic compounds bacterial Furthermore, studies fruit demonstrated efficacy mitigating stimulating fruits, preventing