Effects of Klebsiella michiganensis LDS17 on Codonopsis pilosula growth, rhizosphere soil enzyme activities, and microflora, and genome-wide analysis of plant growth-promoting genes

• DOI: 10.1128/spectrum.04056-23
• Publication Date: 2024-04-02T14:16:22Z
• Authors: Tingting Jin; Jiahong Ren; Bianxia Bai; Wei Wu; Yongqing Cao; Jing Meng; Lihui Zhang
• Keywords: Codonopsis

Codonopsis pilosula is a perennial herbaceous liana with medicinal value. It critical to promote growth through effective and sustainable methods, the use of plant growth-promoting bacteria (PGPB) promising candidate. In this study, we isolated PGPB, Klebsiella michiganensis LDS17, that produced highly active 1-aminocyclopropane-1-carboxylate deaminase from rhizosphere. The strain exhibited multiple properties. antagonistic activity LDS17 against eight phytopathogenic fungi was investigated, results showed had obvious effects on Rhizoctonia solani, Colletotrichum camelliae, Cytospora chrysosperma, Phomopsis macrospore inhibition rates 54.22%, 49.41%, 48.89%, 41.11%, respectively. Inoculation not only significantly increased seedlings but also invertase urease activities, number culturable bacteria, actinomycetes, fungi, as well functional diversity microbial communities in rhizosphere soil seedlings. Heavy metal (HM) resistance tests resistant copper, zinc, nickel. Whole-genome analysis revealed genes involved IAA production, siderophore synthesis, nitrogen fixation, P solubilization, HM resistance. We further identified gene (koyR) encoding plant-responsive LuxR solo genome. may therefore be useful fertilizers for pilosula. identification related provides an important foundation future analyses molecular mechanisms underlying promotion LDS17. comprehensively evaluated characteristics heavy ability strain, inoculation seedling qualities conducted whole-genome lots clusters contributing plant-beneficial functions resistance, which elucidating mechanism expanding its application development agents used environment under stress.