Characteristics of the Salivary Microbiota in Periodontal Diseases and Potential Roles of Individual Bacterial Species To Predict the Severity of Periodontal Disease

• DOI: 10.1128/spectrum.04327-22
• Publication Date: 2023-05-16T14:02:01Z
• Authors: Suk Ji; Joong-Ki Kook; Soon-Nang Park; Yun Kyong Lim; Geum Hee Choi; Jae-Suk Jung
• Keywords: Tannerella forsythia; Prevotella intermedia; Treponema denticola; Forsythia; Fusobacterium nucleatum

The purposes of this study were to examine the compositional changes in salivary microbiota according severity periodontal disease and verify whether distribution specific bacterial species saliva can distinguish disease. Saliva samples collected from 8 periodontally healthy controls, 16 patients with gingivitis, 19 moderate periodontitis, 29 severe periodontitis. V3 V4 regions 16S rRNA gene sequenced, levels 9 showing significant differences among groups by sequencing analysis identified using quantitative real-time PCR (qPCR). predictive performance each distinguishing was evaluated a receiver operating characteristic curve. Twenty-nine species, including Porphyromonas gingivalis, increased as increased, whereas 6 Rothia denticola, decreased. relative abundances P. Tannerella forsythia, Filifactor alocis, Prevotella intermedia determined qPCR significantly different groups. three T. F. alocis positively correlated sum full-mouth probing depth moderately accurate at In conclusion, showed gradual mouth rinse had ability IMPORTANCE Periodontal is one most widespread medical conditions leading cause tooth loss, imposing high economic costs an increasing burden worldwide life expectancy increases. Changes subgingival community during progression affect entire oral ecosystem, bacteria reflect degree imbalance cavity. This explored analyzing suggested biomarkers for saliva.