Fibroblasts from the inner granulation tissue of the pseudocapsule in hips at revision arthroplasty induce osteoclast differentiation, as do stromal cells

• DOI: 10.1136/ard.61.2.103
• Publication Date: 2002-07-27T10:29:07Z
• Authors: H Sakai; S Jingushi; T Shuto; K Urabe; T Ikenoue; K Okazaki; T Kukita; A Kukita; Y Iwamoto
• Keywords: Granulation tissue; Tartrate-resistant acid phosphatase

It has previously been shown that many osteoclast precursors are included in the granulation tissue within pseudocapsule obtained at revision arthroplasty from hips with osteolysis. In vitro culture of only cells isolated to generate mature osteoclasts.To investigate presence or otherwise supporting cells, similar stromal which differentiate osteoclasts tissue.Cells were cultured alone, and after four weeks fibroblast-like (granulation fibroblasts) remained. Rat non-adherent bone marrow (NA-BMCs) co-cultured fibroblasts without 1alpha,25(OH)2D3 (10(-8) M) heat treated ROS 17/2.8 cell conditioned medium (ht ROSCM), both. Multinucleated (MNCs), formed, assessed by biochemical functional characterisation osteoclasts. Receptor activator NFkappaB ligand (RANKL) was investigated immunohistochemistry.Co-culture NA-BMCs caused formation tartrate resistant acid phosphatase (TRAP) positive MNCs, had calcitonin receptor (CTR), Kat-1 antigen, is specific surface rat osteoclasts, ability form pits both ht ROSCM just ROSCM. RANKL detected tissue.These data suggest support differentiation, as do osteoblasts/stromal may play a part aseptic loosening.