A study of HER2 gene amplification and protein expression in gastric cancer

• DOI: 10.1136/jcp.2010.076570
• Publication Date: 2010-08-10T01:55:37Z
• Authors: Benedict Yan; Ee Xuan Yau; Siti Shyba Bte Omar; Chee Wee Ong; Brendan Pang; Khay Guan Yeoh; Manuel Salto-Tellez
• Keywords: CISH; Concordance

Background Gastric cancer is a leading cause of cancer-related mortality, and current treatment outcomes for advanced disease remain poor. HER2 has been identified as potential candidate targeted therapy in gastric cancers displaying gene amplification protein overexpression. Aims To study the prevalence rate amplification/overexpression local population, determine concordance between various modalities. Methods 128 samples were analysed by fluorescence (FISH) chromogenic (CISH) situ hybridisation immunohistochemistry (IHC). The relation status clinicopathological parameters was also analysed. Results 11.7% (15/128) 9.4% (12/128) displayed overexpression (score 3+), respectively, with perfect correlation FISH CISH analyses. There significant inverse overall survival intestinal-type carcinomas (p<0.05). Conclusion Results, besides corroborating existing reports that discrepancies exist ISH IHC assays, suggest need to rigorously evaluate an independent reference standard assessment cancers.