90 Spatio-temporal monitoring of the tumor microenvironment in cutaneous squamous cell carcinoma

• DOI: 10.1136/jitc-2023-sitc2023.0090
• Publication Date: 2023-10-31T13:33:44Z
• Authors: Niyati Jhaveri; Dmytro Klymyshyn; Aditya Pratapa; Bassem Ben Cheikh; James Monkman; Michael Prater; Nadine Nelson; Najiba Mammadova; Arutha Kulasinghe
• Keywords: Multiplex; Immune checkpoint

<h3>Background</h3> Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer, accounting for 75% of deaths due to excluding melanoma. Although prognoses are typically favorable, metastasis and resistance immune checkpoint inhibitors pose an emerging threat. There few biomarkers that can reliably aid in patient stratification, thus multiplex strategies required understand dynamics tumor regulation over course therapy. Here, we aim track microenvironment (TME) inhibitor therapy (ICI). <h3>Methods</h3> Our study investigates spatial TME landscapes cSCC tissues from patients immunotherapy treatment. An ultrahigh-plex antibody panel encompassing lineages, activation states, checkpoints, structural metabolic markers was deployed on PhenoCycler^®-Fusion biology platform analysis tissue microenvironment. Deep performed identify cellular phenotypes, neighborhoods functional states driving pathogenesis response immunotherapy. <h3>Results</h3> data reveals dynamic changes phenotype compositions, signatures In with resistant disease, features were observed, specifically within compartments. Overall, phenotyping landscape novel insights into key molecular determinants clinical outcome. <h3>Conclusions</h3> using a spatio-temporal approach protein-based phenotyping, interrogated pathobiology stratification therapeutic intervention.