Topological reorganizations of mitochondria isolated from rat brain after 72 hours of paradoxical sleep deprivation, revealed by electron cryo-tomography

• DOI: 10.1152/ajpcell.00077.2021
• Publication Date: 2021-05-12T19:50:38Z
• Authors: Zhuoyang Lu; Yachong Hu; Yongyao Wang; Tiantian Zhang; Jiangang Long; Jiankang Liu
• Keywords: Cerebral Cortex; Male; 0301 basic medicine; Electron Microscope Tomography; Sleep, REM; Hippocampus; Mitochondria; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; Oxygen Consumption; Organ Specificity; Mitochondrial Membranes; Image Processing, Computer-Assisted; Animals; Sleep Deprivation

Sleep deprivation has profound influence on several aspects of health and disease. Mitochondria dysfunction been implicated to play an essential role in the neuronal cellular damage induced by sleep deprivation, but little is known about how mitochondrial ultrastructure affected under deprivation. In this report, we utilized electron cryo-tomography reconstruct three-dimensional (3-D) structure extracted morphometric parameters quantitatively characterize its reorganizations. Isolated mitochondria from hippocampus cerebral cortex adult male Sprague-Dawley rats after 72 h paradoxical (PSD) were reconstructed analyzed. Statistical analysis six specific inner membrane topology revealed identical pattern changes both with higher significance levels hippocampus. The structural differences indistinguishable conventional phenotypic methods based two-dimensional microscopy images or 3-D tomography reconstructions. Furthermore, correlate alterations functions, high-resolution respirometry was employed investigate effects PSD respiration, which showed that significantly suppressed respiratory capacity hippocampus, whereas isolated less affected. These results demonstrate capability for quantifying complex reorganizations suggest a correlation between architecture/respiratory functions brain variable different regions.