Acute effects of a ketone monoester, whey protein, or their coingestion on mTOR trafficking and protein-protein colocalization in human skeletal muscle

• DOI: 10.1152/ajpcell.00207.2024
• Publication Date: 2024-04-29T08:04:02Z
• Authors: Sarkis J. Hannaian; Jamie Lov; Zacharie Cheng-Bo...; Sidney Abou Sawan; Nathan Hodson; Benoit J. Gentil; José A. Morais; Tyler A. Churchwa...
• Keywords: 0301 basic medicine; 03 medical and health sciences

We explored the effects of a ketone monoester (KET), whey protein (PRO), or their coingestion (KET + PRO) on mTOR-related protein-protein colocalization and intracellular trafficking in human muscle. All treatments decreased TSC2-Rheb colocalization at 120 minutes; however, KET + PRO sustained the decrease at 300 min. Only PRO and KET + PRO increased mTOR-Rheb colocalization; however, the increase at 300 min was greater in KET + PRO. Treatment intake increased mTOR-WGA colocalization, suggesting translocation to the fiber periphery. Ketone bodies influence the spatial regulation of mTOR.