Human adipocytes from the subcutaneous superficial layer have greater adipogenic potential and lower PPAR-γ DNA methylation levels than deep layer adipocytes

• DOI: 10.1152/ajpcell.00301.2015
• Publication Date: 2016-06-02T03:45:41Z
• Authors: Kentaro Kosaka; Yoshitaka Kubota; Naoki Adachi; Shinsuke Akita; Yoshitaro Sasahara; Tomoe Kira; Masayuki Kuroda; Nobuyuki Mitsukawa; Hideaki Bujo; Kaneshige Satoh
• Keywords: Adult; Leptin; 0301 basic medicine; Adipogenesis; Subcutaneous Fat; Cell Differentiation; DNA Methylation; Middle Aged; PPAR gamma; 03 medical and health sciences; Adipose Tissue; Adipocytes; Humans; CpG Islands; Female; Obesity; Cells, Cultured; Cell Proliferation

Human subcutaneous fat tissue consists of two layers, superficial adipose (SAT) and deep (DAT). Some recent reports suggest that a disproportionate accumulation DAT is related to obesity-associated metabolic complications. However, the differences in adipocyte function between SAT are unclear. To clarify human characteristics DAT, ceiling culture-derived proliferative adipocytes (ccdPAs) were primary cultured from three lean female patients. Differences adipogenic differentiation potential sensitivity exogenous factors examined. Epigenetic modification CpG island DNA methylation levels genes adipogenesis was measured. In histological analyses, mean size significantly larger than (8,741 ± 416 vs. 7,732 213 μm 2 , P < 0.05). Primary showed greater did those DAT. Sensitivity partial stimulation different ccdPAs Peroxisome proliferator-activated receptor-γ (PPAR-γ) protein expression leptin secretion higher PPAR-γ lower Adipocyte vivo. This consistent with findings an vitro study that, compared have PPAR-γ.