Expression of glucocorticoid receptor α- and β-isoforms in human cells and tissues

• DOI: 10.1152/ajpcell.00363.2001
• Publication Date: 2013-05-14T00:10:48Z
• Authors: Laura Pujols; Joaquim Mullol; Jordi Roca-Ferrer; Alfons Torrego; Antoni Xaubet; John A. Cidlowski; César Picado
• Keywords: Alpha (finance)

Alternative splicing of the human glucocorticoid receptor (GR) primary transcript generates two protein isoforms: GR-α and GR-β. We investigated expression both GR isoforms in healthy cells tissues. mRNA abundance (×10 6 cDNA copies/μg total RNA) was as follows: brain (3.83 ± 0.80) > skeletal muscle macrophages lung kidney liver heart eosinophils peripheral blood mononuclear (PBMCs) nasal mucosa neutrophils colon (0.33 0.04). GR-β much less expressed than mRNA. Its 3 (1.55 0.58) PBMCs ≥ (0.15 0.08). not found colon. While detected all tissues, any specimen. Our results suggest that, physiological conditions, default pathway is one leading to GR-α. The alternative event minimally activated.