TREK-2 (K2P10.1) and TRESK (K2P18.1) are major background K+ channels in dorsal root ganglion neurons

• DOI: 10.1152/ajpcell.00629.2005
• Publication Date: 2006-02-23T01:33:49Z
• Authors: Dawon Kang; Donghee Kim
• Keywords: Dorsal root ganglion

Dorsal root ganglion (DRG) neurons express mRNAs for many two-pore domain K + (K 2P ) channels that behave as background channels. To identify functional in DRG neurons, we examined the properties of single-channel openings from cell-attached and inside-out patches cell bodies neurons. We found seven types channels, with conductance ranging 14 to 120 pS 150 mM KCl bath solution. Four these showed biophysical pharmacological similar TRESK (14 pS), TREK-1 (112 TREK-2 (50 TRAAK (73 which are members channel family. The molecular identity three other could not be determined, they low activity were observed infrequently. Of four TRESK-like pS) was most active at 24°C. At 37°C, 50-pS (TREK-2 like) contributed (69%) resting current, followed by 14-pS (16%), TREK-1-like 112-pS (12%), TRAAK-like 73-pS (3%) In all well those TASK-1 TASK-3, expressed, judged RT-PCR analysis. Our results show TREKs together contribute >95% 37°C. As targets modulation receptor agonists, likely play an role regulation excitability