Pregnancy-induced insulin resistance in the rat: assessment by glucose clamp technique

• DOI: 10.1152/ajpendo.1984.246.1.e25
• Publication Date: 2017-12-21T15:49:11Z
• Authors: A. Leturque; A. F. Burnol; P. Ferre; J. Girard
• Keywords: Glucose clamp technique; Clamp; Carbohydrate Metabolism

To quantify and characterize the insulin resistance during pregnancy in rat, a euglycemic hyperinsulinemic clamp was set up. Dose-response curves for effects of five concentrations on glucose production, utilization, clearance were performed age-matched virgin 19-day-pregnant rats. Glucose production utilization measured by using [3-3H]-glucose. totally suppressed at plasma higher than 1,000 microU/ml two groups. Insulin concentration causing half-maximal suppression about 70 rats 250 pregnant Maximal obtained 2,000 microU/ml. In maximal increment significantly lower (P less 0.01) stimulation 200 500 As blood clamped at, respectively, 0.97 +/- 0.03 0.73 mg/ml, rates calculated because this parameter is minimally affected changes concentrations. A normal response to restored but rightward shift dose-response curve maintained. Plasma necessary groups similar that observed when results expressed as utilization. Thus, late rat characterized decreased sensitivity liver peripheral tissues insulin.