Cytokines and endotoxin induce cytokine receptors in skeletal muscle

• DOI: 10.1152/ajpendo.2000.279.1.e196
• Publication Date: 2017-12-22T07:57:27Z
• Authors: Yan Zhang; Geneviève Pilon; André Marette; Vickie E. Baracos
• Keywords: Cytokine receptor; Proinflammatory cytokine; Common gamma chain; Cell surface receptor

Proinflammatory cytokines are important factors in the regulation of diverse aspects skeletal muscle function; however, cytokine receptors mediating these functions uncharacterized. Binding kinetics (dissociation constant = 39+/-4.7 x 10(-9) M, maximal binding 3.5+/-0.23 10(-12) mol/mg membrane protein) tumor necrosis factor (TNF) were obtained. Skeletal was found to express mRNAs encoding interleukin-1 type I and II receptors, interleukin-6 receptor (IL-6R), interferon-gamma by RT-PCR, but below limits detection ligand-binding assay (> or =1 fmol sites/mg protein). Twenty-four hours after intraperitoneal administration endotoxin rats, TNF (TNFRII) IL-6R mRNA increased (P<0.05). In cultured L6 cells, expression TNFRII induced TNF-alpha, all six a mixture IFN-gamma, This suggests that low level is complemented capacity for induction, providing clear mechanism amplification responses at level.