Lumped constant for [18F]fluorodeoxyglucose in skeletal muscles of obese and nonobese humans

• DOI: 10.1152/ajpendo.2000.279.5.e1122
• Publication Date: 2017-12-22T08:42:43Z
• Authors: Pauliina Peltoniemi; Peter Lönnroth; Hanna Laine; Vesa Oikonen; Tuula Tolvanen; Tove Grönroos; Lena Strindberg; Juhani Knuuti; Pirjo Nuutila
• Keywords: Microdialysis; Hyperinsulinemia; Fluorodeoxyglucose

Quantitative 2-[(18)F]fluoro-2-deoxy-D-glucose ([(18)F]FDG) positron emission tomography (PET) has been widely used to calculate glucose utilization in skeletal muscle. FDG-PET results depend partly on the lumped constant (LC), which accounts for differences transport and phosphorylation between [(18)F]FDG glucose. In this study, we estimated LC directly normal insulin-resistant obese subjects by combining FDG PET with microdialysis technique. Eight [age 29.4 +/- 1.0 yr, body mass index (BMI) 33.6 kg/m(2)] eight nonobese (age 25.0 BMI 23.1 kg/m(2)) males were studied during euglycemic hyperinsulinemia (1 mU. kg(-1).min(-1) 150 min). Muscle blood flow was measured using (15)O-labeled water PET. uptake (rGU(FDG)) calculated Patlak graphic analysis. Interstitial concentration of quadriceps femoris muscle simultaneously scanning microdialysis. (by microdialysis, rGU(MD)) multiplying extraction regional flow. A significant correlation found rGU(MD) rGU(FDG) (r = 0.78, P < 0.01). The determined as ratio rGU(MD). averaged 1.16 0.16 similar (1.15 0.11 vs. 0.07, respectively, not significant). conclusion, technique can be reliably combined measure Direct measurements these two independent techniques suggest an value 1.2 human insulin stimulation, appears sensitive resistance.