High-fat diet overfeeding promotes nondetrimental liver steatosis in female mice

• DOI: 10.1152/ajpgi.00022.2018
• Publication Date: 2018-08-10T12:35:54Z
• Authors: Lino Arisqueta; Hiart Navarro-Imaz; Ibone Labiano; Yuri Rueda; Olatz Fresnedo
• Keywords: Steatosis; Enterohepatic circulation; Hepatic lipase; Leptin receptor

High-fat diet (HFD) feeding or leptin-deficient mice are extensively used as models resembling features of human nonalcoholic fatty liver disease (NAFLD). The concurrence experimental factors fat content and source total caloric intake leads to prominent differences in the development hepatic steatosis related disturbances. In this work, we characterized lipid accumulation induced by HFD wild-type (WT) ob/ ob with purpose differentiating adaptations from those specific increased overfeeding due leptin deficiency-associated hyperphagia. Given that most published works have been done male models, female aim increasing body evidence regarding NAFLD subjects. promoted only hyperphagic strain. Nevertheless, a decrease droplet-associated cholesteryl ester (CE) both WT obese animals was observed. These changes were accompanied an improvement profile lipoproteins transport cholesterol function markers plasma lower index. Using primary hepatocytes mice, overaccumulation CE 0.4 mM oleic acid reversed presence Takeda G protein-coupled bile receptor agonist. not responsive. This study suggests enterohepatic circulation acids might be one can affect sex dimorphism development, which underlines importance including research field. NEW & NOTEWORTHY work provides new insight into use high-fat model induce mice. We show induces while, surprisingly, several health indicators improve. Noteworthy, experiments they express it accountable for development.