Myosin 5b is required for proper localization of the intermicrovillar adhesion complex in the intestinal brush border

• DOI: 10.1152/ajpgi.00212.2022
• Publication Date: 2022-10-11T12:00:31Z
• Authors: Sarah A. Dooley; Kristen A. Engevik; Jessica Digrazia; Rachel Stubler; Izumi Kaji; Evan Krystofiak; Amy C. Engevik
• Keywords: Apical membrane; Brush border; Villin

Intestinal enterocytes have an elaborate apical membrane of actin-rich protrusions known as microvilli. The organization microvilli is orchestrated by the intermicrovillar adhesion complex (IMAC), which connects distal tips adjacent IMAC composed CDHR2 and CDHR5 well scaffolding proteins USH1C, ANKS4B, Myosin 7b (MYO7B). To create IMAC, cells must transport to membrane. 5b (MYO5B) a molecular motor that traffics ion transporters enterocytes, we hypothesized MYO5B may also be responsible for localization proteins. address this question, used two different mouse models: 1) neonatal germline knockout (MYO5B KO) mice 2) adult intestinal-specific tamoxifen-inducible VillinCreERT2;MYO5Bflox/flox mice. In control mice, immunostaining revealed CDHR2, CDHR5, MYO7B were highly enriched at contrast, MYO5B-deficient showed loss in brush border accumulation subapical compartment. Colocalization analysis decreased Mander's coefficients inducible compared with MYO7B. Scanning electron microscopy images further demonstrated aberrant packing small intestine. These data indicate delivery components membrane.NEW & NOTEWORTHY intestinal epithelium absorbs nutrients water through organized Microvilli regulated complexes, links between neighboring density. study, report new trafficking partner 5b. Loss results disorganized failure reach