Intestinal serotonin acts as paracrine substance to mediate pancreatic secretion stimulated by luminal factors

• DOI: 10.1152/ajpgi.2001.281.4.g916
• Publication Date: 2017-12-24T16:04:32Z
• Authors: Y. Li; X. Y. Wu; J. X. Zhu; C. Owyang
• Keywords: Male; Serotonin; 0303 health sciences; Dose-Response Relationship, Drug; Parasympatholytics; N-Methylscopolamine; Vagotomy; Rats; Perfusion; Rats, Sprague-Dawley; 03 medical and health sciences; Receptors, Serotonin; Intestine, Small; Paracrine Communication; Animals; Serotonin Antagonists; Capsaicin; Receptors, Serotonin, 5-HT3; Maltose; Pancreas

We recently demonstrated that luminal factors such as osmolality, disaccharides, and mechanical stimulation evoke pancreatic secretion by activating 5-hydroxytryptamine subtype 3 (serotonin-3, 5-HT3) receptors on mucosal vagal afferent fibers in the intestine. hypothesized 5-HT released stimuli acts a paracrine substance, to stimulate secretion. In vivo rat model, perfusion of maltose or hypertonic NaCl increased level threefold intestinal effluent perfusates. Similar levels were observed after intraluminal 10(-5) M perfusion. These treatments did not affect blood levels. separate study, intraduodenal, but intraileal, application induced dose-dependent increase protein secretion, which was blocked CCK-A antagonist CR-1409. Acute vagotomy, methscopolamine, perivagal capsaicin abolished 5-HT-induced conscious rats, administration produced 90% output, markedly inhibited 5-HT3 ondansetron. conclusion, induce release, turn activates terminals. this manner, substance via cholinergic pathway.