Urocortin, a member of the corticotropin-releasing factor family, in normal and diseased heart

• DOI: 10.1152/ajpheart.2000.279.6.h3031
• Publication Date: 2017-12-22T09:13:52Z
• Authors: Toshio Nishikimi; Atsuro Miyata; Takeshi Horio; Fumiki Yoshihara; Noritoshi Nagaya; Shuichi Takishita; Chikao Yutani; Hisayuki Matsuo; Hiroaki Matsuoka; Kenji Kangawa
• Keywords: Urocortin

In the present study we investigated form of expression, action, second messenger, and cellular location urocortin, a member corticotropin-releasing factor (CRF) family, in heart. Urocortin mRNA, as shown by quantitative RT-PCR analysis, is expressed cultured rat cardiac nonmyocytes (NMC) well myocytes (MC) heart, whereas CRF receptor type 2β (CRF-R2β), presumed urocortin predominantly MC compared with NMC. mRNA expression higher left ventricular (LV) hypertrophy than normal LV, CRF-R2β markedly depressed LV LV. more potently increased cAMP levels both NMC did CRF, its effect was potent significantly protein synthesis [ 14 C]Phe incorporations atrial natriuretic peptide secretion collagen DNA 3 H]prolin H]Thy An immunohistochemical revealed that immunoreactivity observed human heart it intense failing These results, together recent evidence for positive inotropic suggest diseased may modulate pathophysiology or at least part, via signaling pathway.