Autophagic response to exercise in peripheral blood mononuclear cells from young men is intensity-dependent and is altered by exposure to environmental heat

• DOI: 10.1152/ajpregu.00110.2022
• Publication Date: 2022-08-22T11:56:49Z
• Authors: James J. McCormick; Melissa D. Côté; Kelli E. King; Morgan K. McManus; Nicholas Goulet; Karol Dokladny; Pope L. Moseley; Glen P. Kenny
• Keywords: Intensity; Exercise intensity

Autophagy is essential to maintaining cellular homeostasis in all eukaryotic cells and tolerance of acute stressors such as starvation, heat, recovery after exercise. Limited information exists regarding the exercise intensity-dependent autophagic response humans, it unknown how environmental heat stress may modulate this response. Therefore, we evaluated autophagy accompanying pathways [the heat-shock (HSR), apoptosis, inflammation] peripheral blood mononuclear (PBMCs) from 10 young men (mean [SD]; 22 [2] years) before, immediately up 6-h postexercise 30 min low-, moderate-, high-intensity semirecumbent cycling [40%, 55%, 70% maximal oxygen consumption (V̇o2max), respectively] a temperate environment (25°C) at V̇o2max hot (40°C). Changes protein content were analyzed via Western blot. Each increase intensity was associated with elevations mean body temperature. LC3-II increased moderate-intensity exercise, further increases (P < 0.05). However, an beclin-2 ULK1, decrease p62 only observed which paralleled by elevated TNF-α cleaved-caspase-3, HSR peaking 6 h When performed greater cleaved-caspase-3 accumulation observed; however, declined our findings indicate that PBMCs during be strain exhibited increasing intensities, modulated exposure heat.