Prolactin 177, prolactin 188, and extracellular osmolality independently regulate the gene expression of ion transport effectors in gill of Mozambique tilapia

• DOI: 10.1152/ajpregu.00168.2015
• Publication Date: 2015-09-17T03:29:15Z
• Authors: Mayu Inokuchi; Jason P. Breves; Shunsuke Moriyama; Soichi Watanabe; Toyoji Kaneko; Darren T. Lerner; E. Gordon Grau; Andre P. Seale
• Keywords: Oreochromis mossambicus; Euryhaline; Osmoregulation

This study characterized the local effects of extracellular osmolality and prolactin (PRL) on branchial ionoregulatory function a euryhaline teleost, Mozambique tilapia ( Oreochromis mossambicus). First, gill filaments were dissected from freshwater (FW)-acclimated incubated in four different osmolalities, 280, 330, 380, 450 mosmol/kg H 2 O. The mRNA expression Na + /K -ATPase α1a (NKA α1a) /Cl − cotransporter (NCC) showed higher with decreasing media while /2Cl 1a (NKCC1a) PRL receptor (PRLR2) levels upregulated by increases osmolality. We then containing ovine (oPRL) native PRLs (tPRL 177 tPRL 188 ). oPRL two tPRLs concentration-dependent NCC, NKAα1a, PRLR1 expression; /H exchanger 3 (NHE3) was increased 24 h incubation tPRLs. Immunohistochemical observation that both maintained high density NCC- NKA-immunoreactive ionocytes cultured filaments. Furthermore, we found differentially induce these ion transporters, according to time. Together, results provide evidence may as osmoreceptors, well directly respond modulate functions.