Candida glabratabinds to glycosylated and lectinic receptors on the coronary endothelial luminal membrane and inhibits flow sense and cardiac responses to agonists

• DOI: 10.1152/ajpregu.00229.2014
• Publication Date: 2015-10-22T03:29:56Z
• Authors: David Torres-Tirado; Maureen Knabb; Irene Castaño; Araceli Patrón-So...; Alejandro De Las ...; Rafael Rubio
• Keywords: Coronary perfusion pressure

Candida glabrata (CG) is an opportunistic fungal pathogen that initiates infection by binding to host cells via specific lectin-like adhesin proteins. We have previously shown the importance of lectin-oligosaccharide in cardiac responses flow and agonists. Because lectinic-oligosaccharide nature CG binding, we tested ability alter agonist- flow-induced changes function isolated perfused guinea pig hearts. Both transmission scanning electron microscopy showed strong attachment coronary endothelium, even after extensive washing. shifted vs. auricular-ventricular (AV) delay relationship upward, indicating greater was required achieve same AV delay. This effect completely reversed with mannose, partially galactose N-acetylgalactosamine, but hyaluronan had no effect. Western blot analysis used determine endothelial luminal membrane (CELM) receptors, results indicate flow-sensitive CELM ANG II type I, α-adrenergic 1A receptor, endothelin-2, VCAM-1 bind CG. In addition, inhibited agonist-induced effects bradykinin, angiotensin, phenylephrine on delay, perfusion pressure, left ventricular pressure. Mannose inhibitory agonist responses. These suggest directly binds receptors interactions mannose disrupts necessary for