In vitro and in vivo effects of ghrelin on luteinizing hormone and growth hormone release in goldfish

• DOI: 10.1152/ajpregu.00669.2003
• Publication Date: 2004-03-08T20:35:03Z
• Authors: Suraj Unniappan; Richard E. Peter
• Keywords: Secretagogue

We studied the in vitro and vivo effects of octanoylated goldfish ghrelin peptides (gGRL-19 gGRL-12) on luteinizing hormone (LH) growth (GH) release goldfish. gGRL-19 gGRL-12 at picomolar doses stimulated LH GH from dispersed pituitary cells perifusion static incubation. Incubation for 2 h with 10 nM 1 or increased LH-beta mRNA expression, whereas only expression. Somatostatin-14 abolished stimulatory culture. The secretagogue receptor antagonist d-Lys(3)-GHRP-6 inhibited ghrelin-induced release, no were found stimulation by ghrelin. Intracerebroventricular injection ng/g body wt intraperitoneal 100 serum levels 60 min after injection, significant increases 15 30 these treatments. Our results indicate that, addition to its potent actions have novel function stimulating